Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZFC3H1 in samples. An antibody specific for ZFC3H1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZFC3H1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFC3H1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFC3H1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: ZFC3H1 May be involved in transcriptional regulation.Contains 1 C3H1-type zinc finger. Contains 6 HAT repeats.Contains 5 TPR repeats.
A zinc finger protein is a DNA-binding protein domain consisting of zinc fingers ranging from two in the Drosophila regulator ADR1, the more common three in mammalian Sp1 up to nine in TFIIIA. They occur in nature as the part of transcription factors conferring DNA sequence specificity as the DNA-binding domain. They have also found use in protein engineering due to their modularity and have prospects as components of tools for use in therapeutic gene modulation and zinc finger nucleases. Zinc finger protein consists of anti-parallel hairpin motif. It consists of 2 beta strands, one alpha helix and a hairpin structure